Personal Statement Of A Fitness App - 1511 Words

As of 2014 almost one third of all smartphone users in the U.S. used a fitness app in the past year and I am one of them (â€Å"Hacking Health†). Last year, I was gifted a Fitbit for Christmas from my father and I began to use it every day along with a calorie counting app called MyFitnessPal. My main goal was to lose weight, and I did. The combination between MyFitnessPal and Fitbit was perfect. I could log my activity with one and what I ate with the other. At my lowest, I had lost close to fifteen pounds and I felt great and looked fabulous in time for my senior prom. After that I felt I no longer needed the help from fitness apps, but as graduation came and went the weight slowly crept back on. Before I knew, it I had gained ten pounds†¦show more content†¦Fitness apps collect multiple different types of personal information and health data such as age, weight, height, body temperature, sleep cycles, activity levels, and so much more. According to Jessica Rich, th e director of the Federal Bureau for Consumer Protection at the Federal Trade Commission, â€Å"Health data from [a person’s] connected device, may be collected and then sold to data brokers and other companies [they] do not know exists† (Liebelson). Lots of popular companies such as Nike, Fitbit, and Garmin collect personal information in databases and use it within their own company to better their products. However, there has been talk that these companies have secretly been selling their data. University of Colorado law school professor, Scott Peppet adds, if not already â€Å"Fitbit will eventually move toward sharing this data† because it â€Å"is so high quality that I could do things like price insurance premiums or I could probably evaluate your credit score incredible accurately† (Liebelson). This should be terrifying to the average consumer because it means Fitbit could easily make top dollar for their health information and they wouldnâ€℠¢t even know about it till it was printed in an article in the New York Times. Not just Fitbit, but any major fitness corporation that has an app could sell information to other companies. Rich says, â€Å"these companies could use [their] information to market other products

Movie Review The Madness Of King George III - 1216 Words

In the film, The Madness of King George, King George III suffers through a deteriorating mental state further exasperated by his declining relationship with his son, the Prince of Wales. The movie takes place in Great Britain during the 1788-1789 Regency Crisis. Realizing his father’s deteriorating mental health, the Prince of Wales aggravates his father’s condition, with the knowledge that he would become regent if the King is found incapable of leading the country. Parliament became divided with the decision. Half wished to make the young George the regent and the other wished to keep the older George as King. The King undergoes the harsh medical treatments of the time and is able to come back to parliament to thwart the regency bill and†¦show more content†¦The directors of the movie used humor to help connect you to the characters and to help lighten the mood of a scary and sad situation. For example, the scene where the King is gallivanting about the palac e in his underclothes playing hide and seek and sliding down banisters provides some comedic relief to the divisive regency crisis occurring just a couple rooms away. The colors of the costumes are bright and regal much like the colors used in the art of the late 1700’s. The colors signify the period in addition to the status of the people within the film. The types of clothes and fabrics that are used, show the wealth of the royals and royal court, in addition to the styles that were commonly worn during this time. In addition to clothing, the power and wealth of England were also shown in the set. The pristine walls of the palace provided a stark contrast to the insane behavior of King George III, further illustrating his mental disability. Nigel Hawthorne’s portrayal of King George is so spot on that it makes any viewer of the film feel as though he is actually insane. This helps the viewers connect with the King even more and really supports his portrayal as the â €Å"good-guy† of the movie. The movie is in favor of King George over his son, the Prince of Wales. The film paints the young George as a conniving power-hungry son, willing to do anything to becomeShow MoreRelatedStephen P. Robbins Timothy A. Judge (2011) Organizational Behaviour 15th Edition New Jersey: Prentice Hall393164 Words   |  1573 Pages1 1 Introduction What Is Organizational Behavior? 3 The Importance of Interpersonal Skills 4 What Managers Do 5 Management Functions 6 †¢ Management Roles 6 †¢ Management Skills 8 †¢ Effective versus Successful Managerial Activities 8 †¢ A Review of the Manager’s Job 9 Enter Organizational Behavior 10 Complementing Intuition with Systematic Study 11 Disciplines That Contribute to the OB Field 13 Psychology 14 †¢ Social Psychology 14 †¢ Sociology 14 †¢ Anthropology 14 There Are Few Absolutes inRead MoreContemporary Issues in Management Accounting211377 Words   |  846 Pagespublished in Behavioral Research in Accounting; Journal of Management Accounting Research; Pacific Accounting Review; Australian Accounting Review; Accounting, Organizations and Society; and Management Accounting Research, as well as contributing chapters to books. In 2002, Jane received the FMAC Articles of Merit Award from IFAC for a co-authored article appearing in the Australian Accounting Review. Stanley Baiman is Ern st Young Professor of Accounting at the Wharton School, University of Pennsylvania

The French Imperfect Essay Sample free essay sample

The Gallic progressive ( imparfait ) is a descriptive yesteryear tense which indicates an on-going province of being or a repeated or uncomplete action. The beginning and terminal of the province of being or action are non indicated. and the progressive is really frequently translated in English as â€Å"was† or â€Å"was ___-ing. † The progressive can bespeak any of the followers: I. Habitual actions or provinces of being Quand j’etais petit. nous allions a la plage chaque semaine. When I was immature. we used to travel to the beach every hebdomad. L’annee derniere. je travaillais avec Monday pere.I worked with my male parent last twelvemonth. II. Physical and emotional descriptions: clip. conditions. age. feelings Il etait Midi et forty-nine faisait boyfriend.It was noon and the conditions was nice. Quand il avait 5 autonomic nervous systems. forty-nine avait toujours faim.When he was five. he was ever hungry. III. Actions or provinces of an unspecified continuance Je faisais La queue parce que j’avais besoin de notes. I stood in line because I needed tickets. Il esperait Te voir avant ton depart.He was trusting to see you before you left. IV. Background information in concurrence with the passe compose J’etais gold marche et j’ai achete des pommes.I was at the market and I bought some apples. Il etait a la banque quand Illinois l’a trouve .He was at the bank when he found it. V. Wishes or suggestions Ah! Si j’etais riche!Oh. if merely I were rich! Si nous sortions ce soir?How approximately traveling out tonight? VI. Conditionss in Si clauses Si j’avais de l’argent. j’irais avec toi.If I had some money. I would travel with you. S’il voulait venir. forty-nine trouverait lupus erythematosus moyen.If he wanted to come. he would happen a manner. VII. The looks etre en train Delawares and venir Delawares in the yesteryear J’etais en train de faire La vaisselle.I was ( in the procedure of ) making the dishes. Il venait d’arriver.He had merely arrived. Gallic has two verbs which can be translated by the English verb â€Å"to know† : savoir andconnaitre. This can be confounding to English talkers. but in fact there are distinguishable differences in significance and use for the two verbs. Savoir has three possible significances: 1 ) to cognize a fact Je sais qu’il l’a fait.I know he did it. 2 ) to cognize by bosom Je sais cette nouvelle ( par c?ur ) .I know this short narrative ( by bosom ) . 3 ) to cognize how to make something ( note that the word â€Å"how† is non translated into French ) Savez-vous conduire?Make you cognize how to drive? Je ne sais pas nager.I don’t cognize how to swim. In the passe compose . savoir means â€Å"to learn† or â€Å"to find out† : J’ai su qu’il l’a fait.I found out that he did it. Savoir is frequently followed by a subsidiary clause. Je sais ou Illinois Eastern Time.I know where he is. Il sait que nous serons a la fete.He knows we’ll be at the party. Connaitre has two significances: 1 ) to cognize a individual Je connais Pierrette.I know Pierrette. 2 ) to be familiar with a individual or thing Je connais bien Toulouse.I know / am familiar with Toulouse. Je connais cette nouvelle – je l’ai lue l’annee derniere. I know / am familiar with this short narrative – I read it last twelvemonth. In the passe compose . connaitre means â€Å"to meet ( for the first clip ) / become acquainted with† : J’ai connu Pierrette a Lyon.I met Pierrette in Lyon. Connaitre ever needs a direct object: Je connais boy poeme.I am familiar with his verse form. Je connais bien ton pere.I know your male parent good. Nous connaissons Paris.We know/are familiar with Paris. Il La connait.He knows her. Ignorer is a related verb which means â€Å"not to know† in the sense of â€Å"to be incognizant of. † Depending on the context. it can replace either ne pas savoir or ne pas connaitre. J’ignore quand Illinois arrivera.I don’t cognize when he is geting. Il ignore Ionesco.He’s non cognizant of ( doesn’t know about ) Ionesco.By and large talking. the imperfect describes past state of affairss. while the passe composenarrates specific events. In add-on. the progressive can put the phase for an event expressed with the passe compose . Compare the utilizations of these two tenses: 1. Incomplete vs Complete The progressive describes an on-going action with no specified completion: J’allais en France. – I was traveling to France. Je visitais des memorials et prenais diethylstilbestrols photos. – I was sing memorials and taking images The passe compose expresses one or more events or actions that began and ended in the yesteryear: Je suis alle en France. – I went to France. J’ai visite des memorials et pris diethylstilbestrols photos. – I visited some memorials and took some images. 2. Accustomed V Occasional The progressive is used for accustomed or perennial actions. something that happened an countless figure of times: Je voyageais en France tous lupus erythematosuss autonomic nervous systems. – I traveled ( used to go ) to France every twelvemonth. Je visitais souvent lupus erythematosus Louvre. – I frequently visited the Louvre. The passe compose negotiations about a individual event. or an event that happened a specific figure of times: J’ai voyage en France l’annee derniere. – I traveled in France last twelvemonth. J’ai visite lupus erythematosus Louvre trois fois. – I’ve visited the Louvre three times. 3. Ongoing vs New The imperfect describes a general physical or mental province of being: J’avais peur diethylstilbestrols chiens. – I was afraid of Canis familiariss. J’aimais les epinards. – I used to like Spinacia oleracea. The passe compose indicates a alteration in physical or mental province at a precise minute or for an stray cause: J’ai eu peur quand le chien a aboye . – I was scared when the Canis familiaris barked. Pour La premiere fois. j’ai aime les epinards. – For the first clip. I liked Spinacia oleracea. 4. Background + Interruption The progressive and passe compose sometimes work together – the progressive provides a description/background info. to put the scene of how things were or what was go oning ( past tense of â€Å"be† + verb with -ing normally indicates this ) when something ( expressed with the passe compose ) interrupted. J’etais a la banque quand Chirac est arrive . – I was at the bank when Chirac arrived. Je vivais en Espagne quand je l’ai trouve . – I was populating in Spain when I found it. IntroductionUses of the passe compose and progressiveCompare transitions and discover key words and phrasesTrial: Passe compose Vs Imperfect Note: There is a 3rd tense. the passe simple. which technically translates to the English simple past tense. but is now used chiefly in composing. in topographic point of the passe compose – learn more. Once you understand the different utilizations of the progressive and passe compose as explained on page 2. take a expression at these transitions to compare how these two Gallic past tenses may be used. Progressive Quand j’avais 15 autonomic nervous systems. je voulais etre psychiatre. Je m’interessais a la psychologie parce que je connaissais beaucoup de names tres bizarres. Le week-end. j’allais a La bibliotheque et j’etudiais pendent toute La journee. When I was 15. I wanted to be a head-shrinker. I was interested in psychological science because I knew a batch of truly eldritch people. On the weekends. I used to travel to the library and analyze all twenty-four hours. Passe compose Un jour. je suis tombe malade et j’ai decouvert les miracles de la medecine. J’ai fait La connaissance d’un medecin et j’ai commence a etudier avec lui. Quand la faculte de medecine m’a accepte . je n’ai plus pense a La psychologie. One twenty-four hours. I got ill and discovered the admirations of medical specialty. I met a physician and started analyzing with him. After the medical school accepted me. I didn’t think about psychological science any more. IndexsThe undermentioned key words and phrases tend to be used with either the progressive or the passe compose . so when you see any of them. you know which tense you need: Imperfect| Passe compose|chaque semaine. mois. annee | every hebdomad. month. year| une semaine. un mois. un an| one hebdomad. month. year| le week-end| on the weekends| un week-end| one weekend| lupus erythematosus lundi. le mardi†¦| on Mondays. on Tuesdays†¦ | lundi. mardi†¦| on Monday. on Tuesday| tous lupus erythematosuss jours| every day| un jour| one day| lupus erythematosus soir| in the evenings| un soir| one evening|toujours| always| soudainement| suddenly|normalement| usually| tout a putsch. tout d’un putsch | all of a sudden| d’habitude| usually| une fois. deux fois†¦| one time. twice†¦| en general. generalement| in general. generally| enfin| finally| souvent| often| finalement| in the end| parfois. quelquefois| sometimes| plusieurs fois| several times| de temps en temps| from clip to cliprarement| seldomautrefois| onceNotes: Some Gallic verbs are used chiefly in the progressive. while others have different significances depending on which tense they are used in. Learn more about advanced yesteryear tenses. There is a 3rd tense. the passe simple. which technically translates to the English simple past tense. but is now used chiefly in composing. as the literary equivalent of the passe compose . | The jussive mood. called l’imperatif in French. is a verb temper which is usedto * give an order* express a desire* make a petition* offer advice* recommend somethingUnlike all other Gallic verb tenses and personal tempers. the capable pronoun is non used with the jussive mood: Fermez La Porte.Near the door. Mangeons maintenant.Let’s eat now. Ayez la bonte de m’attendre.Please delay for me. Veuillez m’excuser.Please pardon me. The above are called â€Å"affirmative bids. † because they are stating person to make something. â€Å"Negative bids. † which tell person non to make something. are made by puting Ne in forepart of the verb and the appropriate negative adverb after the verb: Ne parle pas!Don’t speak! N’oublions pas les livres.Let’s non bury the books. N’ayez jamais peur.Never be afraid. Avocations – Sports – GamesPasse-temps favoris – Sports – JeuxHere are the Gallic names of some common avocations. athleticss. and games. You can utilize these nouns with verbs like aimer and detester. or if you want to state that you do or play that point. utilize it with the verb in the 3rd column. The 4th column has verbs that can be used entirely to speak about making that point. e. g. . cuisiner – to cook. English| French| Use with| Or use| basketball| lupus erythematosus basket| jouer a| |biking| lupus erythematosus cyclisme. le velo| faire de| |chess| les echecs| jouer a| |cooking| La cuisine| faire| cuisiner|dancing| La danse| | danser|fishing| La peche| aller a| pecher|football| lupus erythematosus football americain| jouer a| |gardening| lupus erythematosus jardinage| faire de| jardiner|hiking| La randonnee| faire de| |hockey| lupus erythematosus hockey| jouer a| |hunting| La chasse| faire| chasser|jogging| lupus erythematosus jogging| faire de| |a movie| un film| regarder| |music| La musique| ecouter. jouer de| |reading| La lecture| faire| lire|sailing| La voile| faire| |skiing| lupus erythematosus ski| faire de| skier|soccer| lupus erythematosus football. le foot| jouer a| |swimming| La natation| faire de| nager|telecasting ( Television ) | la television ( la tele ) | regarder| | tennis| lupus erythematosus tennis| jouer a| |wrestling| La lutte| faire de| lutter| The Gallic adverbial pronouns Y is so bantam that one might believe its function in a sentence is non really of import. but in fact rather the antonym is true. It is highly of import in Gallic. Y refers to a antecedently mentioned or implied topographic point ; it is usuallytranslated by â€Å"there† in English. Y normally replaces a prepositional phrase get downing with something like a. chez. ordans. Are you traveling to the bank today? No. I’m traveling ( there ) tomorrow. Tu vessel a La banque aujourd’hui? Non. j’y vais demain. We’re traveling to the shop. Do you desire to travel ( at that place ) ?Nous allons au magasin. Tu veux Y aller? He was at Jean’s house. He was at that place.Il etait chez Jean. Il y etait. Note that â€Å"there† can frequently be omitted in English. but y can neer be omitted in French. Je vais ( I’m traveling ) is non a complete sentence in French ; if you don’t follow the verb with a topographic point. you have to sayJ’y vais. Yttrium can besides replace a + a noun that is non a individual. * such as with verbs that need a . Note that in French. you must include either a + something or its replacing y. even though the equivalent may be optional in English. You can non replace the noun with an object pronoun. I’m reacting to a missive. I’m reacting ( to it ) .Je reponds a une lettre. J’y reponds.Incorrect: Je reponds. Je la reponds. Je lui reponds. He’s believing about our trip. He’s believing about it.Il pense a notre ocean trip. Il y pense.Incorrect: Forty-nine pense. Il le pense. Il lui pense. You have to obey the jurisprudence. You have to obey it.Tu Department of the Interior obeir a La loi. Tu Department of the Interior Y obeir.Incorrect: Tu dois obeir. Tu Department of the Interior l’obeir. Tu Department of the Interior lui obeir. Yes. I attended the meeting. Yes. I attended ( it ) .Oui. j’ai assiste a La reunion. Oui. j’y ai assiste . Incorrect: Oui. j’aiassiste . je l’ai assiste . je lui ai assiste . I’m traveling to believe about your proposal. I’m traveling to believe about it. Je vais reflechir a votre proposition. Je vais Y reflechir. Incorrect: Je vais reflechir. Je vais La reflechir. Je vais lui reflechir. *In most instances. a + individual may merely be replaced by an indirect object. However. in the instance of verbs that don’t allow predating indirect object pronouns. you can utilize Y: Pay attending to him.Fais attending a lui. Fais-y attending.Incorrect: Fais-lui attending. Note that y normally can non replace a + verb. I hesitate to state the truth. I hesitate to state it.J’hesite a dire La verite . J’hesite a la dire.Incorrect: J’y hesite. I continue to read Balzac. I continue to read him.Je continue a lire Balzac. Je continue a lupus erythematosus lire.Incorrect: J’y continue. The Gallic adverbial pronoun nut is so bantam that one might believe its function in a sentence is non really of import. but in fact rather the antonym is true. It is highly of import in Gallic. En replaces the partitive article + noun or de + indefinite article + noun. It is tantamount to â€Å"some. † â€Å"any. † or â€Å"one† in English. Do you hold any staff of life? Yes. I have some.As-tu du hurting? Oui. j’en Army Intelligence. He wants an apple. He wants one.Il a envie d’une pomme. Il en a envie. I don’t need an helper. I don’t need one.Je n’ai pas besoin d’un adjutant. Je n’en Army Intelligence pas besoin. In a sentence with a qualifier. such as an adverb of measure or a figure. plus noun. en replaces the noun and the qualifier or figure is placed at the terminal of the sentence. Note that â€Å"of it† and â€Å"of them† are normally optional in English. but en is required in French. There are a batch of suites. There are a batch ( of them ) .Il y a beaucoup de chambres. Il Y en a beaucoup. I don’t have adequate money. I don’t have adequate ( of it ) . Je n’ai pas assez d’argent. Je n’en Army Intelligence pas assez. I’d like two books. I’d like two ( of them ) .Je voudrais deux livres. J’en voudrais deux. We bought 10 notebooks. We bought 10 ( of them ) .Nous Avons achete 10 cahiers. Nous en Avons achete 10. En besides replaces de + noun with verbs and looks that need de. Again. in Gallic. you must include either de + something or its replacing nut. even though â€Å"about/of it† is normally optional in English. What do you believe about my thought? What do you believe ( about it ) ? Que penses-tu de mon idee? Qu’en penses-tu?Incorrect: Que penses-tu? What are the effects of this determination? What are the effects ( of it ) ? Quelles sont lupus erythematosuss consequences de cette decision? Quelles en sont lupus erythematosuss consequences? Incorrect: Quelles sont lupus erythematosuss consequences? Note that en normally can non replace de + verb. I decided to accept his offer. I decided to accept it. J’ai decide d’accepter boy offre. J’ai decide de l’accepter. Incorrect: J’en ai decide . I forgot to rinse the auto. I forgot to rinse it.J’ai oublie de laver la voiture. J’ai oublie de la laver. Incorrect: J’en ai oublie . Note that nut is besides a preposition.

QR Codes Overview

Ochman (2013) argues that QR codes are being replaced by other applications which are easier to use. These applications come in form of invisible ink and they are more technically superior to QR codes.Advertising We will write a custom critical writing sample on QR Codes Overview specifically for you for only $16.05 $11/page Learn More The past QR codes used to record contact information before directing a web user to a website. New applications have advanced features which allow users to browse their smart phones and access displayed content directly. This has improved users’ experience because these apps are easily customised to reflect users’ needs and lifestyles. These apps direct users to different types of content they like, which makes them use less time to locate what they want to read or watch. QR codes require a user to scan them using a camera on their hand held devices, which puts off some users. Some of these codes are displaye d in inaccessible areas, making it difficult for users to scan them. There are new apps which allow users to scan special sections of articles and are becoming popular with online newspaper readers. Tokyo Shimbu, a Japanese newspaper, uses the AR News App, which allows users to scan specific articles using smartphones. This application has encouraged newspapers to attract young readers by customizing content to appeal to their interests. The app allows children to find suitable content that appeals to them because it is clearly marked. Another popular app, Blippar allows users to get information and other entertaining content, which is displayed in 3D. Touchcode is an app with an invisible code, which allows users to confirm whether a brand is original or not.Advertising Looking for critical writing on other technology? Let's see if we can help you! Get your first paper with 15% OFF Learn More These apps are making QR codes obsolete, even though they have been used for the past two decades. In the past, QR codes had good applications that made it possible for users to buy music, through its mobile-code campaign (Ochman, 2013). However, these codes were displayed in areas that were difficult to scan, which discouraged many users from trying them. There are technical issues, which affect the usage of QR codes in different environments. They have security risks, which discourage users from using them. The codes need to be simplified to encourage more users to try them out. Companies that display information through QR codes need to explain to users how they add value to them. QR codes should be displayed in areas that are easily accessible to make them convenient to customers. QR codes platforms need to be made more secure to reduce security threats which users are exposed to. Some users are directed to malicious content and websites whenever they scan these codes. This makes it harder for them to locate specific websites they are looking for. Other applications offer more convenience to users and this has created a shift in usage patterns. Some QR code readers have functional problems, which makes them unable to capture accurate content. Patrizio (2012) reveals they lack certified standards, which makes it difficult to monitor their performance.Advertising We will write a custom critical writing sample on QR Codes Overview specifically for you for only $16.05 $11/page Learn More Smart phone manufacturers have not been given specifications they need to observe to make their QR readers more responsive to users’ needs. However, QR codes are used more by retail sellers to track their produce, to find out where it has come from. Experts feel that companies which use QR codes need to make them more secure to ensure users are not directed to inappropriate websites. However, QR codes are essential because they allow users to get crucial information on a wide variety of subjects on their smartphone s, after scanning. References Ochman, B.L. (2013, March 26). QR codes are dead, trampled by easier-to-use apps. Ad Age Digital. Web. Patrizio, A. (2012, September 18). QR codes pose security risk, becoming obsolete experts claim. Technology Guide. Web. This critical writing on QR Codes Overview was written and submitted by user Liam Richardson to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

Free Essays on Milk

Why Milk Matters for Teens In my opinion, we don't get enough milk at lunch. I usually get 3 or 4 milks at lunch which requires me to spend more money on lunchs. One funny fact is that we have had the same sized milks since elementry school. Here are some useful facts about calcium and teens.Good nutrition is important for good health and can help protect against many diseases later in life. However, one important nutrient many teens don't get enough of is calcium, found mainly in milk and dairy products and in dark green, leafy vegetables and foods with added calcium. Calcium is a nutrient that helps to make bones and teeth strong and healthy. It is used in building bone mass and also helps to reduce the risk of bone fracture due to osteoporosis, a condition where bones become fragile and can break easily. Accordding to the Health and Nutrition Examination Survey, Females 9-19 years old only get 19% of the milk they need, and males 9-19 years old get 52% of the milk they need. Our bodies continually remove and replace small amounts of calcium from our bones. If your body removes more calcium than it replaces, your bones will become weaker and have a greater chance of breaking. But by getting the recommended amount of calcium, you can help your bones stay strong. Teens and young adults, ages 9-18, need more calcium because their bones are growing more than at other times of life. They should have 1,300 mg of calcium per day, or about 3 servings of Milk Group foods daily. One 8-ounce glass of milk has about 300 mg of calcium, so just a few glasses can go a long way towards getting the calcium needed each day. Low-fat and fat-free milk and dairy products, such as cheese and yogurt, are excellent sources of calcium. In addition to having lots of calcium, milk and dairy products provide other essential nutrients, all necessary for good bone health and development. These include phosphorus, magnesium, and added vitamin D in... Free Essays on Milk Free Essays on Milk Why Milk Matters for Teens In my opinion, we don't get enough milk at lunch. I usually get 3 or 4 milks at lunch which requires me to spend more money on lunchs. One funny fact is that we have had the same sized milks since elementry school. Here are some useful facts about calcium and teens.Good nutrition is important for good health and can help protect against many diseases later in life. However, one important nutrient many teens don't get enough of is calcium, found mainly in milk and dairy products and in dark green, leafy vegetables and foods with added calcium. Calcium is a nutrient that helps to make bones and teeth strong and healthy. It is used in building bone mass and also helps to reduce the risk of bone fracture due to osteoporosis, a condition where bones become fragile and can break easily. Accordding to the Health and Nutrition Examination Survey, Females 9-19 years old only get 19% of the milk they need, and males 9-19 years old get 52% of the milk they need. Our bodies continually remove and replace small amounts of calcium from our bones. If your body removes more calcium than it replaces, your bones will become weaker and have a greater chance of breaking. But by getting the recommended amount of calcium, you can help your bones stay strong. Teens and young adults, ages 9-18, need more calcium because their bones are growing more than at other times of life. They should have 1,300 mg of calcium per day, or about 3 servings of Milk Group foods daily. One 8-ounce glass of milk has about 300 mg of calcium, so just a few glasses can go a long way towards getting the calcium needed each day. Low-fat and fat-free milk and dairy products, such as cheese and yogurt, are excellent sources of calcium. In addition to having lots of calcium, milk and dairy products provide other essential nutrients, all necessary for good bone health and development. These include phosphorus, magnesium, and added vitamin D in...

Professional Communication Definition and Issues

Professional Communication Definition and Issues The term professional communication refers to the various forms of speaking, listening, writing, and responding carried out both in and beyond the workplace, whether in person or electronically. From meetings and presentations to memos and emails to marketing materials and annual reports, in business communication, its essential to take a professional, formal, civil tone to make the best impression on your audience, whether its members be your colleagues, supervisors, or customers. Author Anne Eisenberg illustrates it this way:  What is good professional communication? It is writing or speaking that is accurate, complete, and understandable to its audience- that tells the truth about the data directly and clearly. Doing this takes research, analysis of the audience, and the mastering of the three interrelated elements of organization, language, and design and illustration. (Writing Well for the Technical Professions. Harper Row, 1989) Even if youre comfortable with your coworkers, you should still take the extra time to make your emails among them professional, correct, and clear. Becoming too lazy or informal in them (with grammar, punctuation, and spelling, for example) can reflect poorly on you if a message would happen to be forwarded to higher levels of the company or to human resources. Always keep them cordial, and reread for potential misunderstandings before you hit send. Social Media Reflects on Your Brand With a bevy of social media avenues representing your (and your companys) public face, its critical that the communications presented there represent you well.   Author  Matt Krumrie elaborates:  For professionals, their brand shows through on their LinkedIn photo and profile. It shows through with your e-mail signature. It shows on Twitter by what you tweet and through your profile description. Any form of professional communication, whether it’s intended to or not, reflects your personal brand. If you attend a networking event, how you present yourself is how people perceive you and your brand. (Can a Personal Brand Coach Help My Career?  Star Tribune  [Minneapolis], May 19, 2014) Remember that whats sent in an email or posted on the Internet is very tough to completely delete, and if its been saved by someone (such as in a forward or retweet), its possible it wont ever completely go away. Have others review what you plan to post, not only for typos and factual errors but for potential cultural  insensitivity. Even be careful of what you post on your personal sites and pages, as they can come back to haunt you professionally, especially if you deal with the public or customers in your job- or someday will want a job that does.   Intercultural Communication One issue in todays global, interconnected economy is the potential for miscommunication when dealing with people of other cultures if employees are not sensitive to the norms of people that they have to interact with- and a company doesnt have to be dealing with people across the globe for this to apply. Even people from across the United States have different ways of communicating. Someone from the South or Midwest might find the bluntness of a New Yorker off-putting, for example. Intercultural communication is communication between and among individuals and groups across national and ethnic boundaries, notes authors  Jennifer Waldeck, Patricia Kearney, and Tim Plax. It can also come up in rural vs. urban or generational divides. They continue: Intercultural communication can become especially problematic for business communicators when they begin to believe that the way people in their dominant culture communicate is the only or best way, or when they fail to learn and appreciate the cultural norms of people they do business with. (Business and Professional Communication in a Digital Age. Wadsworth, 2013) Fortunately, companies have a wealth of resources available to them under the umbrella of sensitivity training. Working with a diverse set of colleagues can help everyone understand others perspectives. Tap into your colleagues to learn their points of view and prevent gaffes in your communications before they happen.

Issue In Financial Reporting Essay Example | Topics and Well Written Essays - 1500 words

Issue In Financial Reporting - Essay Example In accordance to the general purpose of financial reporting, financial reports are used to provide the users with information about the reporting entity. The report that will be submitted by the Management Committee of RVCC will serve as a tool for the users for their evaluation since they are supported, given grants, and funded by some other entities (like that of Commonwealth Government and Queensland State Government). The financial reports well serve also transparencies for everybody to inform where all the money goes. If RVCCC will be able to submit the financial reports to their users, it will give the users ideas if the funds allocation or resources allocation they gave are efficient. In other words, this will gain the trust as well for RVCCC from their supporters and funding entity (Review of Business, 2005). The reports will also monitor the cash flows and will also serve as bases to their expenses. It is also well stated in the Definition of Reporting Entity (2001), that all they are entities like them are required to submit financial reports especially to public sectors that helps them, like that of the commonwealth Government and Queensland State Government. ... 2: For each of the following independent cases, discuss whether you believe the items mentioned would meet the definition and recognition criteria for the various elements as mentioned in the 'Framework for the Preparation and Presentation of Financial Statements (ie the AASB Framework). (Ensure that you provide reasons to support your answers in each case and state clearly any assumptions you have made in preparing your answers.): (a) Tate Retailers Ltd operates a chain of retail stores that specialize in the sale of rugby league products. Due to an economy-wide downturn, the company has decided to reduce staffing levels by offering voluntary redundancy packages to 200 of its employees. After agreement was reached with the unions, letters were sent to all employees shortly before the end of the reporting period inviting applications for the package. A response was required within 30 days, which was after the end of the reporting period. The company's board of directors estimates that the redundancies will cost the company $2 million. The financial controller of the company, Bob Johnson, is considering whether to record a provision for redundancies to account for the future redundancy payments to staff. Would this account satisfy the liability definition criteria in the AASB Framework Would this transaction meet the recognition criteria for liabilities according to the AASB Framework (Normally you would not con sider the recognition criteria if the definition for liabilities was not met. However, for the purposes of this assignment you should address the recognition criteria regardless of whether or not you determine that the definition criteria have been satisfied.) - This account shall satisfy the liability definition criteria in the ASBB Framework. Their employees

Literature Review and Report (The Woolworths Management Success Story) Essay

Literature Review and Report (The Woolworths Management Success Story) - Essay Example This literature review analyzes the problems associated with overworking employees and provides alternative solutions. When employees work beyond a normal 40 hour week people sometimes develop an addiction to work. These types of employees are workaholics. According to a study performed at the University of North Carolina about one-fourth of the American workforce suffers from â€Å"workaholism† which is defined as a compulsive need to work at the expense of everything else in one’s life (All the Work and no Play Makes Your AP Staff Less Productive, 2008). When people put in extra hours at work it does not necessarily mean they are being more productive. These workers are typically exhausted from the excessive work load. They become more inefficient as a consequence and it takes them longer to accomplish tasks. Giving your employees excessive work tasks is not a sound business strategy and is actually counterproductive. In the United States businesses lost nearly $150 billion last year due to overworking their employees (All the Work and no Play Makes Your AP Staff Less Productive, 200 8). Overworking employees is detrimental to both the company and to the health of the employees. Overworked employees developed health issues such as panic attacks, headaches, tiredness, fatigue, sleeping disorders, irritability, and chronic stress among others. These health issues many times cause employees to lose work days due to sickness. When employees are overworked they lose motivation in the workplace and their job performance suffers. Overworking create a hostile business environment in which the employees feel consumed by the long hours they have to put in everyday. Employees that suffer from work related stress are susceptible to weight loss and to developing bad habits such as caffeine and nicotine addiction (News from Personnel Today, 2009). These addictions cause the employees to take extra

Employment - Management Essay Example for Free

Employment Management Essay For the longest time I could not decide on a major and a career to study throughout college. When I came to Georgia Southern University I discovered that they had a major that was very interesting to me. Sport Management id the ideal major for my interest and me. Since I love sports I figured that this would be the career for me. A degree in Sport Management helps to prepare for success in sport related occupations. The job market varies in the field of sport management. Some occupational opportunities include: athletic trainer, coach, sports official, Sports agent, camp director, sporting goods sales/dealer, pro scout, athletic director, sport promoter. The list can go on of the opportunities in this career. Mostly anything sport related in todays business world is included also. The nature of a person in a sport management career is based solely around sports. Depending on what occupation you decide to pursue the work and conditions will differ. Some typical activities are, plan and direct athletic events, represent professional athletes, plan and direct the training of the team players, evaluate skills and potential of players, or work extensively with players, coaches, officials, managers etc. The work condition can vary with different jobs or tasks. A scout will be called on to travel about 3/4 of the time. Athletic directors handle the athletics of their prospective schools along with coaches. A sports agent working conditions can involve a lot of long hours and extended pressure. Some employment settings are colleges/universities, camps, sporting goods stores, management firms, professional teams, fitness centers and the media. The job outlook for most careers in Sport Management is fair to good. Sport Management is one of the fastest growing fields of study in the country. With that there will lots of job openings and new businesses starting. Some jobs in this field are limited. For instance, anything dealing with professional teams is limited due to the number of sports teams. Successes in the teams help with salary and benefits for the employees. Most jobs in the sport management field are setup to where an employee must work his or her way up the ladder. For example, the job may ask the employee to assist in work and the salaries are not as high. In some jobs such as being a general manager of a professional team the job is to an extent being in the right place at the right time. Careers in sport management require some necessary in order to be successful. Being able to communicate effectively is a very important skill in this career. Giving Speeches is one part of communication that is important. Decision Making, Organizing, Leading/Coordinating and being able to motivate others is also critical in the career. Qualifications for most jobs require a bachelors degree, sport experience and management training.

Relationships of Waverly Jong and Jing-mei Woo in The Joy Luck Club Ess

The Relationships of Waverly Jong and Jing-mei Woo in The Joy Luck Club  Ã‚        Ã‚   Amy Tan in her novel The Joy Luck Club presents us with daughters who are striving to place themselves beyond the control of strong mothers and become individuals. Adrienne Rich in her book Of Woman Born calls this splitting from the mother, "matraphobia" (Rich, 235), and later notes: "The mother stands for the victim in ourselves, the unfree woman, the martyr. Our personalities seem dangerously to blur and overlap with our mothers; and, in a desperate attempt to know where mother ends and daughter begins we perform radical surgery." (Rich, 236) Tan shows us two characters in her novel who consciously split from their mother when they feel unable to claim their true selves. These two characters are Waverly Jong and Jing-mei Woo.    Waverly's break from her mother comes when she perceives her mother's pride in her ability to play chess being something that increases her mother's own self worth. Waverly tells us: "I knew it was a mistake to say anything more, but I heard my voice speaking. 'Wh...

Woman in Black

Why do you think Susan Hill called her story The Woman in Black? How effective is it as a title? One of the most important parts of any book can be the title and in this case, Susan Hill has decided on her title as being The Woman in Black. Throughout this essay I will be looking at how Susan Hill has used her title effectively and I will also look at the reasons why Hill might have named her title The Woman in Black.At first it may seem a very simple title but as I will go through in this essay, simple might be the very cause of the effect it makes. Firstly, the story itself is heavily based on a gothic genre which consists of all the things you would expect to see in a gothic novel, haunted houses, isolated areas, dark themes and of course, in this particular novel, this mysterious ‘Woman in Black’. With this gothic genre, comes a gothic title which is definitely identified by one word, â€Å"Black†.The work ‘black’ always creates a negative mood an d shows darkness and links this mood with the story, so you know what sort of things to expect from this book just from the title but more importantly, you want to know why. This is what will encourage the reader to read and enjoy the book. Secondly, I think a sense of mystery is created by the title ‘The Woman in Black’ and this creates questions about the book. This adds to the enjoyment of the book as their will always be tension and suspense in anticipation about the appearance of the Woman in Black.An example of this is when Arthur Kipps calls the woman in at the funeral â€Å"the woman with the wasted face†, this would just seem normal but the fact that this woman is all in black relates us back to the title which creates suspicion about this woman. The main questions I think the title brings up about this book is who actually is this Woman in Black and why is she so important in the book? And these questions will stir around in your head the throughout the novel and will add to the effect that the Woman in Black brings as all will be focused on her.Overall, I think the title ‘The Woman in Black’ forms a better interest for the novel in a variety of different ways and all these different ways, however small or large their effect, build up and create the effect as a whole. I think the effect is most noticeably shown through the entertainment of the novel which the title adds towards as well and the main reason I think Susan Hill named her novel this title was to enhance the entertainment that this novel produces. Woman in Black Why do you think Susan Hill called her story The Woman in Black? How effective is it as a title? One of the most important parts of any book can be the title and in this case, Susan Hill has decided on her title as being The Woman in Black. Throughout this essay I will be looking at how Susan Hill has used her title effectively and I will also look at the reasons why Hill might have named her title The Woman in Black.At first it may seem a very simple title but as I will go through in this essay, simple might be the very cause of the effect it makes. Firstly, the story itself is heavily based on a gothic genre which consists of all the things you would expect to see in a gothic novel, haunted houses, isolated areas, dark themes and of course, in this particular novel, this mysterious ‘Woman in Black’. With this gothic genre, comes a gothic title which is definitely identified by one word, â€Å"Black†.The work ‘black’ always creates a negative mood an d shows darkness and links this mood with the story, so you know what sort of things to expect from this book just from the title but more importantly, you want to know why. This is what will encourage the reader to read and enjoy the book. Secondly, I think a sense of mystery is created by the title ‘The Woman in Black’ and this creates questions about the book. This adds to the enjoyment of the book as their will always be tension and suspense in anticipation about the appearance of the Woman in Black.An example of this is when Arthur Kipps calls the woman in at the funeral â€Å"the woman with the wasted face†, this would just seem normal but the fact that this woman is all in black relates us back to the title which creates suspicion about this woman. The main questions I think the title brings up about this book is who actually is this Woman in Black and why is she so important in the book? And these questions will stir around in your head the throughout the novel and will add to the effect that the Woman in Black brings as all will be focused on her.Overall, I think the title ‘The Woman in Black’ forms a better interest for the novel in a variety of different ways and all these different ways, however small or large their effect, build up and create the effect as a whole. I think the effect is most noticeably shown through the entertainment of the novel which the title adds towards as well and the main reason I think Susan Hill named her novel this title was to enhance the entertainment that this novel produces.

Maths

GCSE Mathematics Specimen Papers and Mark Schemes For first teaching from September 2010 For first examination in Summer 2011 For first award in Summer 2012 Subject Code: 2210 Foreword The awarding bodies have prepared new specifications to comply with revised GCSE criteria. The specimen examination papers accompanying new specifications are provided to give centres guidance on the structure and character of the planned examinations in advance of the first examination.It is intended that the specimen papers and mark schemes contained in this booklet will help teachers and students to understand, as fully as possible, the markers’ expectations of candidates’ responses to the types of questions set at GCSE level. These specimen papers and mark schemes should be used in conjunction with CCEA’s GCSE Mathematics specification. GCSE Mathematics Specimen Papers and Mark Schemes Contents Specimen Papers Unit T1 Mathematics (Foundation Tier) Unit T2 Mathematics (Foundatio n Tier) Unit T3 Mathematics (Higher Tier) Unit T4 Mathematics (Higher Tier) Unit T5 Mathematics (Foundation Tier) Paper 1Unit T5 Mathematics (Foundation Tier) Paper 2 Unit T6 Mathematics (Higher Tier) Paper 1 Unit T6 Mathematics (Higher Tier) Paper 2 1 3 23 43 63 83 93 107 121 Mark Schemes General Marking Instructions Unit T1 Mathematics (Foundation Tier) Unit T2 Mathematics (Foundation Tier) Unit T3 Mathematics (Higher Tier) Unit T4 Mathematics (Higher Tier) Unit T5 Mathematics (Foundation Tier) Paper 1 Unit T5 Mathematics (Foundation Tier) Paper 2 Unit T6 Mathematics (Higher Tier) Paper 1 Unit T6 Mathematics (Higher Tier) Paper 2 133 135 137 143 149 157 163 167 171 175 Subject Code QAN 2210 500/7925/6A CCEA Publication  © 2010 You may download further copies of this publication from www. ccea. org. uk SPECIMEN PAPERS DIVIDER PAPER FRONT 1 SPECIMEN PAPERS DIVIDER PAPER BACK 2 Centre Number 71 Candidate Number General Certificate of Secondary Education 2011 Mathematics For Examine r’s use only Question Marks Number Unit T1 (With calculator) Foundation Tier [CODE] SPECIMEN EXAMINATION PAPER TIME 1 hour 30 minutes INSTRUCTIONS TO CANDIDATES Write your Centre Number and Candidate Number in the spaces provided at the top of this page. Write your answers in the spaces provided in this question paper.Answer all twenty five questions. Any working should be clearly shown in the spaces provided since marks may be awarded for partially correct solutions. You may use a calculator for this paper. INFORMATION FOR CANDIDATES The total mark for this paper is 100. Figures in brackets printed down the right-hand side of pages indicate the marks awarded to each question or part question. Functional elements will be assessed in this paper. Quality of written communication will be assessed in questions 6 and 23. You should have a calculator, ruler, compasses and a protractor.The formula sheet is overleaf. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 T otal Marks 3 Foundation Tier Formulae Sheet Area of trapezium = 1 (a + b)h 2 Volume of prism = area of cross section ? length 4 Answer all questions 1 (a) Write 80% as a decimal Answer _____________ [1] Answer ___________ % [1] Answer_____________________ [1] Answer_____________ [1] Answer_____________ [1] (b) Write 0. 35 as a percentage (c) Write 48 million in figures (d) 5729 people attended a football match. Write the number 5729 to (i) the nearest 10 (ii) the nearest 100 2 (a)Find the next 2 terms in the sequence and explain the rule you used: 6, 11, 16, 21, _____, ______ Rule _________________________________________________ [3] (b) Find the next term in the sequence 0. 2, 0. 4, 0. 8, 1. 6, _______ [1] 5 3 The diagram shows a tiled patio in the shape of a rectangle 3 by 16, covered with 48 square tiles. Write down the length and width of 2 other possible rectangles which can be covered with 48 of these square tiles. Answer__________ by__________ __________ by__________ 4 [1] [1 ] Michael recorded the colours of cars in the school car park in a tally chart. ColourTally Frequency Red |||| 4 Blue || 2 Yellow ||| Black |||| || White |||| |||| Silver |||| Green |||| (a) Complete the frequency column. [1] (b) On the grid opposite, draw a frequency diagram to show this information. [3] 6 (c) What is the most popular colour of car in the car park? Answer_________________ (d) [1] Using the frequency table, write down the fraction of the total cars which are yellow. Answer_________________ 7 [1] 5 (a) (i) Shade the major segment in the circle below [1] (ii) (b) PQ is called a _________________ of the circle. (i) Shade the minor sector in the circle below. 1] [1] (ii) OS is called a _______________ of the circle. 8 [1] 6 The table below shows the percentage of pupils at a High School who obtained a grade C or better in GCSE Mathematics during the past five years. Year % of pupils (a) 2004 75 2005 78 2006 82 2007 84 2008 90 Which year showed the smallest improvement? Answer______________ (b) [1] Your quality of written communication will be assessed in this question The school wants to show this information using a statistical diagram. Which type of diagram would you use? Answer__________________________ [1] Give a reason for your answer. _______________________________________________________________ ________________________________________________________________ 7 [2] Here is a list of numbers 25 27 32 35 8 21 9 (a) From the list write down those numbers which are (i) multiples of 5 Answer____________ (ii) [1] Answer____________ [1] factors of 54 9 (b) From the list of numbers (i) calculate the mean Answer_____________ Answer_____________ (ii) 8 [2] [2] find the median In a mid season sale a clothing shop has 20% off all its items. Clare bought a dress which originally cost ? 50 and a hat which originally cost ? 25 (a)How much did she save in the sale? Answer ? _____________ Answer ? _____________ (b) 9 [2] [1] Answer_____________ [2] What wa s her total bill? Simplify 5p ? 2r ? 3p + 5r 10 10 (a) Jo bought 6 roses at 67p each. What change did she get from a ? 5 note? Answer ? _____________ (b) Five kilograms of potatoes and two kilograms of onions cost ? 4. 10 in total. The potatoes cost 62p per kilogram. How much would it cost in total to buy one kilogram of potatoes and one kilogram of onions? Answer ? _____________ 11 [2] [4] The brick shown below is in the form of a cuboid, measuring 6. 4 metres by 3. metres by 2. 6 metres. Calculate the volume of the brick. Answer_____________ 11 [3] 12 Calculate (a) the square root of 1. 44 Answer_____________ Answer_____________ (e) 13 [2] Answer_____________ (d) [1] Answer_____________ (c) [1] Answer_____________ (b) [1] [2] the cube of 2. 8 2. 32 ? 1. 69 3 of 125 5 5. 62 ? 3. 4 The table below gives the maximum and minimum temperatures of six different cities in Europe in March. City Belfast Minimum 2 ° C Dublin ?1 ° C 9 ° C London 4 ° C 16 ° C Edinburgh 0 ° C 11 ° C Barcelona 10 ° C 19 ° C 8 ° C 20 ° C Paris (a) Maximum 10 ° C Which minimum temperature was the lowest?Answer____________________ ° C 12 [1] (b) In two of these cities the temperatures had increased from minimum to maximum by 12 ° C. Write down the names of these two cities. Answer____________________ and ____________________ [2] What is the difference in minimum temperature between Dublin and Paris? (c) Answer_____________ ° C 14 [1] Answer_______________ [1] Answer_____________ % [1] Answer_____________ % [1] Results of a Year 12 Physics test 9 8 7 6 5 4 2 0 2 7 4 6 Key 5 4 (a) 5 1 5 8 6 7 6 8 9 7 9 8 9 9 means 54% How many pupils sat the Physics test? (b) What is the modal percentage mark? c) What is the range of percentage marks? 13 15 The diagram shows the plan for a rectangular garden. Calculate (a) the area of the garden Answer____________m2 [2] Answer____________m2 [2] (b) the area of the plot for the trees A border needs to be dug around the perimeter of the garden. (c) Calculate the perimeter of the garden. Answer____________m 14 [2] 16 The diagram shows a pizza which has been divided into 8 equal parts. The shaded parts are eaten. (a) Write down, as a fraction in its lowest terms, the fraction that is eaten. Answer_____________ Answer___________ % (b) 17 [2] [1]What percentage is left uneaten? Which fractions from the list given below are not equivalent to 2 ? 3 8 10 16 4 12 , , ,, 12 15 28 6 16 Answer_____________ 15 [2] 18 In a survey 300 men were asked which sport they liked best. The pie-chart below shows the results. (a) Measure the angle which represents Basketball. Answer_____________? (b) [1] What fraction of men chose Rugby as their favourite sport? Answer_____________ (c) [1] Answer_____________ [2] How many men chose Hurling as their favourite sport? 16 19 (a) Expand 3(x + 1) Answer______________ [2] Answer_____________ [2] (b) Solve 2y + 3 = 19 0 In the diagram the point P (? 4, 4) has been plotted. (a) Plot the follow ing points on the diagram, labelling clearly Q (? 2, ? 3), R (5, ? 3) and S (3, 4) [3] (b) Join up the points in order and name the quadrilateral formed. Answer____________________ 17 [1] 21 (Diagram not drawn accurately) Calculate (a) x x = ___________? [1] y = ___________? [1] (b) y 22 Draw the net of the matchbox tray (no lid) shown in the diagram, which has base 5cm by 3cm and height 2cm, on the square grid provided. [3] 18 23 Your quality of written communication will be assessed in this question Fred has just won ? 00. 1 1 of it to his son, James. He has promised of it to his daughter, Kathy and 5 4 How much will he have left after he gives Kathy and James their shares? Show clearly each step of your working out. Answer ? _____________ 19 [4] 24 The positions of two towns A and B are shown on the grid. (a) A third town C is 3km east and 2km north of A. Using a scale of 1cm = 0. 5km, show the position of C. (b) [2] How far is C from A? Answer_____________km 20 [3] 25 The follow ing information shows how Sinead spends her time on a Saturday. Activity Cleaning Watching TV Number of hours 2 Using Shopping he Exercising Internet 5 4 3 2 Sleeping 8 Draw a pie chart to illustrate this data. [4] 21 ___________________________________________ THIS IS THE END OF THE QUESTION PAPER ___________________________________________ 22 Centre Number 71 Candidate Number General Certificate of Secondary Education 2011 Mathematics Unit T2 (With calculator) Foundation Tier [CODE] SPECIMEN EXAMINATION PAPER TIME 1 hour 30 minutes INSTRUCTIONS TO CANDIDATES Write your Centre Number and Candidate Number in the spaces provided at the top of this page. Write your answers in the spaces provided in the question paper.Answer all twenty three questions. Any working should be clearly shown in the spaces provided since marks may be awarded for partially correct solutions. You may use a calculator for this paper. INFORMATION FOR CANDIDATES The total mark for this paper is 100. Figures in b rackets printed down the right-hand side of pages indicate the marks awarded to each question or part question. Functional Elements will be assessed in this paper. Quality of written communication will be assessed in questions 5 and 17. You should have a calculator, ruler, compasses and protractor. The formula sheet is overleaf.For Examiner’s use only Question Marks Number 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Total Marks 23 Foundation Tier Formulae Sheet Area of trapezium = 1 (a + b)h 2 Volume of prism = area of cross section ? length 24 Answer all questions 1 Five kilograms of potatoes and two kilograms of onions cost ? 4. 10 in total. The potatoes cost 62p per kilogram. How much would it cost in total to buy one kilogram of potatoes and one kilogram of onions? Answer ? _____________ 2 Answer_____________ (a) [4] [2] Answer_____________ [2] Simplify 5p ? 2r ? 3p + 5r (b) Expand ?2(2y ? 3) 25 3 Calculate a) the cube of 2. 8 Answer______________ Answer__ ____________ (b) (c) [1] [1] Answer______________ [2] 2. 32 + 1. 69 5. 62 ? 3. 4 26 4 Results of a Year 12 Physics test 9 8 7 6 5 4 2 0 2 7 4 6 Key 5 4 (a) 5 1 5 8 6 7 6 8 9 7 9 8 9 9 means 54% How many pupils sat the Physics test? Answer_____________ (b) [1] What is the modal percentage mark? Answer___________% Answer___________% (c) 5 [1] [1] What is the range of percentage marks? Quality of written communication will be assessed in this question Fred has just won ? 900 1 1 He has promised of it to his daughter Kathy, and of it to his son James. 4 How much will he have left after he gives Kathy and James their shares? Show clearly each step of your working out. Answer ? ______________ 27 [4] 6 The positions of two towns A and B are shown on the grid. (a) A third town C is on a bearing of 120? from B and at a distance of 2. 5 km from B. Using a scale of 1 cm = 0. 5km, show the position of C. [3] (b) How far is C from A? Answer _____________ km [2] 28 7 The following information sho ws how Sinead spends her time on a Saturday. Activity Cleaning Watching TV Shopping Number of hours 2 5 4 Using the Exercising Internet 3 2 Sleeping 8Draw a pie chart to illustrate this data. [4] 8 Solve (a) x = 15 4 Answer x = _____________ (b) [1] Answer y = _____________ [2] 6y ? 2 = 13 29 9 Write down the next two numbers in the sequence 11, 10, 8, 5, ____, _____ Answer________, _________ 10 [2] In the diagram the volume of the cuboid is 48cm3. It holds exactly 48 sugar cubes each 1cm by 1cm by 1cm. The length of the cuboid is 4cm and the breadth is 3cm. (a) What is the height of the cuboid? Answer_____________ (b) Write down the dimensions of another cuboid that the 48 cubes could fit into exactly. Answer______cm by______cm by______cm 1 (a) [3] Find the value of [1] 3. 8 ? 6. 2 giving your answer correct to 1 decimal place. 9. 1 ? 2. 7 Answer_____________ 30 [2] (b) A plasma TV has a marked price of ? 790 In a sale its price is reduced by 15% What is the sale price of the TV? A nswer ? _____________ (c) [3] Mary’s family drink 3 cartons of orange juice in 5 days. How many cartons would Mary need to buy to last a full week? Answer _____________cartons 12 [3] Write down an expression for the total cost of x bars of chocolate at 35p each and y bottles of water at 50p each. Answer_______________________ 31 [2] 13Draw the graph of y = 4x–3 on the grid below. [3] 14 (Diagram not drawn accurately) The quadrilateral shown has angles x, 79? , 3x, and 97? Work out the value of x Answer x = _________________o 32 [4] 15 (a) (Diagram not drawn accurately) In the triangle ABC shown above BC = 8. 5 cm and AX = 6. 4 cm. Calculate the area of the triangle ABC. Answer___________________cm2 [2] (b) (Diagram not drawn accurately) ABCDE is a regular pentagon, with O as its centre. Calculate the size of angle AOB. Answer Angle AOB = _____________? 33 [2] 16 Find the area of a circle with a diameter of 3 metres.Take ? = 3. 14 Answer___________________m2 17 (a) [2] The speeds, in miles per hour, of the cars passing the gates of a primary school during lunch hour are recorded in the table below. Speed (mph) No. of cars 0–5 2 6–10 5 11–15 34 16–20 61 21–25 29 26–30 4 Represent this information using a bar chart. [3] 34 (b) Which is the modal class interval? Answer________________ (c) [1] Your quality of written communication will be assessed in this question Katy wants to know how many times a month, on average, the people in her town go to the cinema. She asks 200 people in her school.Explain why Katy’s sample may not be representative of the people in her town. _______________________________________________________________ _______________________________________________________________ 35 [2] 18 (a) Write 72 as a product of prime factors Answer______________ (b) [2] Find the lowest common multiple (LCM) of 72 and 108 Answer______________ Answer______________ (c) 19 [2] [2] Find the highest comm on factor (HCF) of 72 and 108 Susan puts ? 1700 in her bank account at 4. 2% simple interest each year. Calculate the total amount in her bank account after 3 years. Answer ? ________________ 36 [3] 0 (a) Expand and simplify 4(2 – 3x) + 3(x + 4) Answer________________ Answer________________ [2] Answer x =________________ (b) [2] [3] Answer________________ [2] Expand x ( x 2 – 6) (c) Solve for x 7x + 18 = 2(x – 6) 21 (a) ? A regular polygon has an exterior angle of 18 Find the number of sides in the polygon. 37 (b) The diagram shows a play tent in the shape of a triangular prism. Calculate the volume of the tent. Answer_____________________cm3 38 [6] 22 A teacher recorded the number of hours 50 students used the internet over a 7 day period. The information is shown in the table below. Number of Hours 0? h

Free sample - Political Science. translation missing

Political Science. Political ScienceFreedom and political freedom for that matter refers to the lack of interference with the supreme, independent authority over an area or even territory by individual(s) through use of coercion or aggression. A state is said to be free if her government does not oppress her people. A society which is not free is the said to be a totalitarian society and is characterized by absence of political freedom with an aim to regulate almost every aspect of behavior. With such a society, freedom would refer to human relations only and coercion is a common phenomenon (Phillips, 276). The concept of political freedom is closely associated with civil liberties and individual rights. Political freedom is not a standalone concept but an aggregation of many concepts and freedoms which include; freedom of assembly, freedom of association, freedom of movement, freedom of religion, freedom of speech, freedom of press, freedom of thought, freedom from unreasonable seizure, scientific fre edom and academic freedom. All these freedoms shape the political freedom for it is the nature of the politics of any country that they are allowed or even denied (Friedrich A., 84). While freedom is more on the sovereignty of any state and her people, democracy refers to a government of the people, by the people for the people. Democracy can direct where the power to govern the people is directly from the people or it can be representative democracy where people elect or choose representatives to join the authorities on the behalf of the people (Brown H., 44). The United States of America has had a complex political system than most of the other government systems in the world. This is associated with the social divisions in the United States of America. The United States of America has both whites and black Americans. The coexistence of these two groups has not been very friendly. The whites always see the blacks as a lesser gene that incapable of doing what the whites can do. This kind of a division and oppression is seen on almost all the key sectors of the United States Economy from political world, government appointments and even business. As a result, the blacks have struggled their way out of poverty and even if little has been achieved, there is some light at the end of the tunnel. For instance, due to oppression, most blacks went into the music industry where they felt they can influence a large number of people especially the youth. Their music is mostly rap and the message is fighting the oppressing authorities. The whites rule the United States of America. The ruling class has gone further more and above any other in the world to suppress any public discussion about the social classes in the USA. Anticommunist groups in the late 1940 campaigned to outlaw any public opinions on socialism, Marxism among other classes in the United States. Those behind the move were the likes of John McCarthy. The public did not take it lightly and instead responded with fury. It resulted to the 1960 civil unrest in the United States of America. The government put some efforts to frustrate any efforts by the civil society to liberate itself through divide, confuse and rule. Things went out of hand and the American working class started emerging as an independent social force. Other groups like the human rights groups, labor movements and gay liberation all emerged. There was a large division of the society between the small group of capitalist who owned the production factors and was mostly the whites and the majority of the population who must sell their labor capabilities to make a living who were mostly blacks. With those characteristics of the United States of America and its economy, freedom had no meaning especially fir the oppressed and they to fight for it. The Democratic Party became the vehicle to liberation especially for the blacks. Oppressions were along the lines of race and gender. The party recruited blacks, females and Hispanic politicians. With these efforts, the last twelve years of the United States of America has been under two Democratic presidents and one republican with the post of United States secretary of state being given to white man, black man. This can be seen as a step forward towards the achieving freedom through fighting. The biggest hurdle to clear remains not on the racial ground but on the economic division. All these leaders are representatives of ruling class of the US and the world for that matter but not for blacks or white. Barack Obama on being elected the president of the United States of America crowned the fight. Also the dream of martin Luther Kin g jnr. came into fulfillment that one day there will be a more united society in USA where blacks and whites will be treated equally. He is the first African American president and he has overseen the biggest hand over of resources to the rich and Wall Street speculator in history of the United States of America.   He has also promoted nationwide wage cutting and union busting and in this Obama has demonstrated that he is out to serve not to be bonked by his skin color or even origin. United States of America still faces the challenge of a gap along economic endowments with 1 per cent of the population owning 40 per cent of the US wealth and monopolizing about 20 per cent of the total US income. Affirmative action in the public sector Affirmative action is a policy by the government with an aim to fight imbalances and injustices within its citizens. The injustices result unfairness and lack of a level ground for the players. This has been the case in the United States of America. Initially it was along racial biases where the blacks could not mix with the whites; some jobs were reserved for the whites, the big estates and cities were seen to belong to the whites. This is how the northern America has developed to a first world while countries in the Southern America have remained struggling and even today are classified as second world and they are mostly occupied by the blacks. Currently there are biases along economic grounds where the rich rule America and make decisions. Affirmative action would correct these anomalies. The policy though difficult to implement for those in authority and the rich will fight it, will ensure that there is a balance between the whites and the blacks in government appointments among other white collar jobs. Women should be equally represented as well as men. According to the protection clause of the 14th amendment, every citizen needs to be given an equal opportunity. Citizens should not be exposed to unfairness. For this to be realized then affirmative action is inevitable for under normal and real circumstances the level ground cannot be achieved for some people in the same economy are so much ahead of the others with resources and political power and hence the government needs to   put some checks and balances.    References Brown, Charles H., the Correspondents' War. New York: Charles Scribers' Sons, 2005. Friedrich August von Hayek, ‘Freedom and Coercion’ in David Miller (ed), Liberty (2007) pp. 80-86 Patrick Martin (2009). The fundamental social division is class, not race or gender.Phillips Shively, Power and Choice: An Introduction to Political Science. 8th ed. NY: McGraw Hill, 2003.

Warm-Up Activities and Fillers for the French Classroom

Warm-Up Activities and Fillers for the French Classroom Most language teachers find that there is a bit of dead time during class. This may occur at the beginning of class, as the students are arriving; at the end of class, as they are thinking about leaving; and right in the middle of class, when transitioning from one lesson to another. During this dead time, the best option is to spend five or ten minutes on a short, interesting activity. Teachers from all over have shared some great ideas for warm-up and filler activities- take a look. Building Sentences Put together the parts of a sentence. Categories List all of the vocabulary in a particular category. Conversations Pair off for short discussions. Meet Your Neighbor Practice greetings and personal details with other students. Music Videos Watch and discuss French music videos. Name Game Learn all of the students names. Quotations Discuss quotations by famous Francophones. Repetitions Have students repeat a list of vocabulary.

Performence and reward answers Essay Example | Topics and Well Written Essays - 750 words

Performence and reward answers - Essay Example als include â€Å"high volunteer retention level†, the â€Å"where the company’s going† it could do that by defining appropriate processes, the â€Å"how the company’s going to get there†. These could include award ceremonies to recognize and reward good performers, or set policies on how to conduct appraisals of performance. Finally, the â€Å"Content Level†, or the â€Å"what† needed to achieve the goal, for these could simply include managing difficult behavior of the volunteers or setting their goals. This approach is followed by companies throughout the world, including Microsoft. In the review process, a rater or raters evaluate the performance of the employees. All employees are given goals and targets to achieve. These expected results are compared with the actual results and the individual employee is evaluated based on the targets he could achieve or perhaps, exceed. This performance appraisal is a good source of motivation for employees when salary increases, bonuses, etc. are tied with it. It builds up a sense of trust between the employees and the employers and encourages employees to work harder and more efficiently. (Bauer & Erdogan, 1969) However, it is also possible that the appraisal process backfires. If it is not done accurately and the right kind of work and employees are not appreciated, employees would be highly frustrated and de-motivated resulting in dissatisfaction, low morale, and high turnover rate. (Bauer & Erdogan, 1969) There are theories that relate to the needs that employees have and it is important for managers and organizations as whole to understand these needs and cater to them. The Abraham Maslow’s Hierarchy of Needs Theory talks about the five differnet levels of needs for individuals and how these needs act as motivators for them (Wagner). The five levels, starting from the bottom, are psychological needs, safety needs, social needs, esteem needs and self-actualization. Once a level is achieved by an individual, those

File Management Essay Example | Topics and Well Written Essays - 1000 words

File Management - Essay Example This essay focuses on analyzing and describing of modern file management and it's systems that refers to the fundamental methods used in naming, storing and handling computer files. In many applications today, a file acts as a central element, with the exception of the real-time and other specialized applications where, virtually, all output is saved within a file to provide for long term storage for future use by either the user or other programs. The lifespan for the files is independent of any applications that use them for their input-output functions. Therefore, users must be in a position to create access, save and maintain the files content and integrity. These actions encompass the file management systems. A typical file management system, that is discussed in the essay comprises of system utility programs running as privileged applications. Files are manageable in different ways based on platform and the type of file manager. Despite there being different kinds of file manag ers, effective file management must entail proper organization, management and location of directories, files and folders. In summary, file manager enhances locating the files, organizing, storing and backing up files. In organizing the files, the applications are made easier to find through creation of the program file. It is suggested that the documents with same file extensions can be saved in their own folders to facilitate easier and quick retrieval. The user can create nested folders within the folders to prevent orphans and widows.

Has Americas foreign policy been successful in the Middle East Research Paper

Has Americas foreign policy been successful in the Middle East - Research Paper Example The foreign policy of America represents the ways of interacting with different overseas nations of the world along with setting standards for interacting with the individual citizens, corporations and organizations. The prime goal of the US foreign policy is to establish a secure, prosperous and democratic world, which would serve beneficial for the people of America and others as well as who belong to various international countries. In this context, the US has separated foreign policies for the Middle East countries that determine its relation in terms of carrying out effective trade and foreign relations with the same. The strategic interest of the US over the Middle East countries is the major reason behind the formulation and the execution of the foreign policies. Justifiably, Middle East nations have huge deposits of oil and therefore acquiring this product is the main strategic interest of the US. The US foreign policy for the Middle East gained its significance after the end of World War II. During the Cold War, the American foreign policy aimed at preventing the anti-communist regimes of Soviet Union towards the Arab countries (Dow Jones & Company, 2015). However, after the attack of 9/11 in the year 2001, the US foreign policy started concentrating on addressing and mitigating anti-terrorism related activities (Johnson, 2015). The US possesses diplomatic relations with different Middle East countries apart from Iran, as this nation once went with anti-American regime in the year 1979 (Chomsky, 2010).

Blastocystis Hominis and Colorectal Cancer

Blastocystis Hominis and Colorectal Cancer Blastocystis hominis (B.hominis) is the most common unicellular protozoan parasite that is found in the human gastrointestinal tract (Windsor et al. 2002). The prevalence of B. hominis in both developed and developing countries is reported to be up to 10% and 50% respectively (Wong et al. 2008; Stenzel and Boreham. 1996). On the other hand, B. hominis shows a diverse morphologies which include vacuolar, granular, amoeboid and cyst forms (Zierdt. 1988). Furthermore, B. hominis isolates from human and animals have been reported to exhibit an extensive genetic and karyotypic heterogeneity (Parkar et al. 2010; Stensvold et al. 2009; Tan et al. 2009; Dogruman-Al et al. 2009; Abe. 2004; Yoshikawa et al. 2004). Although many researchers have attempted to identify the pathogenesis of B. hominis in human hosts, however the pathogenic potential of B. hominis has remained controversial as it is present in both symptomatic and asymptomatic individual (Tan. 2008; Garcia. 2007; Tan et al. 2002). The gastrointestinal symptoms that are associated with B. hominis infection include diarrhea, vomiting, anorexia, flatulence, abdominal discomfort and other associated non-specific symptoms. To date, experiments have shown that rats that are inoculated with human B. hominis isolate have demonstrated an elevation in oxidative damage (Chandramathi et al. 2009). Many studies have been done on the genetic and molecular characterization of B. hominis isolates derived from human as well as animal. Recent molecular characterization of Blastocystis isolates suggested that there are 13 subtypes (ST1-ST13) which have been isolated from mammalian, reptilian, avian and amphibian hosts (U. Parkar et al. 2010; Stensvold et al. 2007a; Noà «l et al. 2005, 2003; Yoshikawa et al. 2004b). Recently, many studies have been done to determine the pathogenic potential of different Blastocystis subtypes in human hosts by genotypic analysis as well as molecular characterization of B. hominis isolated from symptomatic and asymptomatic individuals (Dominguez-Mà ¡rquez et al. 2009; Eroglu et al. 2009; Jones et al. 2009; Hussein et al. 2008; Tan et al. 2008). Nevertheless, only limited numbers of studies have shown correlation between the different B. hominis subtypes on the pathogenesis in human hosts (Hussein et al. 2008). Infectious agents such as bacteria, virus and parasites, often have an oncogenic potential. The International Agency on Research of Cancer (IARC) has estimated that 16% of cancer worldwide is caused by infection, including parasites. However, there has been no research done on the association between B. hominis infection and colorectal cancer. A recent study has suggested that B. hominis may possess the ability to induce the growth of colorectal cancer cell lines by inhibiting the apoptotic effect of colon cancer cells. Furthermore, the antigens that are isolated from B. hominis were postulated to be able to promote the proliferation of cancer cells via down-regulation of host immune cellular responses (Chandramathi et al. 2010). In general, inflammation is activated by a variety of stimuli such as trauma, bacterial/viral/parasitic infections, endotoxemia and heating (Ley, 2001; Hart, 2002). Inflammation caused by infectious agents such as parasites will cause an increase in the production of reactive oxygen species (ROS) such as nitric oxide, hydrogen peroxide and superoxide as a consequence of cell mediated phagocyte dependent immune response (Rosen et al. 1995). A persistent and chronic inflammatory response can be detrimental to human host as it can produce a chronic damage by releasing a variety of pro-inflammatory and anti-inflammatory cytokines which then leads to mutagenesis, carcinogenesis, neurodegenerative disorder, inflammatory bowel syndrome and atherosclerosis (Kà ¼hn et al. 1993; Perry et al. 1998; Ludewig et al. 2002; Shacter et al. 2002). Several studies have shown correlation between the inflammation that is caused by infectious agents such as parasites and the development of cancer in huma n (Fitzpatrick. 2001). Thus, it is important for us to evaluate the immunomodulation, cytopathic and cellular cytokines responses as a result of B. hominis infection especially in colorectal carcinomas. Since B. hominis is often present in most stool cultures, it is pertinent to investigate the association between B. hominis infection and the development of colorectal cancer in the gastrointestinal tract of infected host system. B. hominis isolated from an asymptomatic individual could facilitate the proliferation and growth of cancer cells and has the potential to down-regulate the host immune response (Chandramathi et al. 2010). However, only limited numbers of studies have shown the cytopathic effects and cellular cytokine responses of Blastocystis hominis infection (Puthia et al. 2008; Long et al. 2001; Walderich et al. 1998). These studies only investigated on Interleukin 8 and Granulocyte-macrophage colony stimulating factor (GM-CSF) which are pro-inflammatory cytokines. We hypothesized that B. hominis infection in human host system has a potential carcinogenic effect and could influence the growth of colorectal cancer cells especially in colorectal cancer patients and that the B. hominis subtypes may exert varying degree of pathogenecity. Therefore, in this study, the differences between the effects of solubilized antigen of B. hominis from both symptomatic and asymptomatic isolates on the cell viability in the peripheral blood mononuclear cells (PBMCs, which represent the immune cells) as well as colorectal cancer cell line was evaluated. Besides that, the gene expression of cytokine, apoptotic mediators, and nuclear transcriptional factors in response to the symptomatic and asymptomatic B. hominis antigen in both PBMCs and colorectal cancer cell was compared. The knowledge and understanding in the association between Blastocystis hominis infection and colorectal cancer as well as the role of various cytokines involved in the tumour progression may provide an insight into prevention and/or development of new immune-therapeutical strategies to combat colorectal cancer. OBJECTIVE The objectives of the present study are:- To study the effects of solubilized antigen of B. hominis from symptomatic and asymptomatic individual isolates on viability of both the peripheral blood mononuclear cells (PBMCs, which represent the normal immune cells) as well as colorectal cancer cells (HCT 116, colorectal cancer cell line). To investigate the gene expression of cytokine, transcriptional factors and apoptotic mediators such as interleukin 6, interleukin 8, tumour necrosis factor-ÃŽ ±, interferon gamma, nuclear factor kappa light chain enhancer of activated B cells, cathepsin B, transforming growth factor-ÃŽ ², and protein 53 upon exposure to B. hominis antigen in both the PBMCs and colorectal cancer cells MATERIALS AND METHODS Sample collection and axenization of Blastocystis hominis B. hominis was isolated from stool samples of symptomatic as well as asymptomatic individuals. The B. hominis cysts were then isolated from feacal sample using the Ficoll-Paque Technique according to Zaman and Khan (1994). The harvested cysts of B. hominis from both the symptomatic and asymptomatic individual isolates were washed in sterile phosphate-buffered saline (PBS). The washed harvested cysts were cultured in Jones medium, supplemented with 10% of heat-inactivated horse serum and incubated at 37 °C in CO2 incubator (Suresh and Smith. 2004). Axenization was conducted after the isolates have been incubated for 2 to 3 days. Isolation of solubilized antigen from symptomatic and asymptomatic cultures The axenic B. hominis species were collected using Ficoll-Paque density gradient centrifugation method and the harvested parasites were resuspended in basal Jones medium without the addition of heat-inactivated horse serum. Then, the harvested organisms were lysed by sonication method and the homogenate was incubated at 4 °C, overnight. The homogenate was centrifuged at 13000xg for 15 minutes at 4 °C after the overnight incubation. The supernatant which contains the solubilized antigen of B. hominis was filter-sterilized and the protein concentration of the antigen was determined using Bradford assay (Bio-Rad, USA). Subtyping of Blastocystis isolates from symptomatic and asymptomatic cultures Genomic DNA Extraction: The genomic DNA of symptomatic and asymptomatic isolates of Blastocystis hominis were extracted from the 3 to 4 days old culture with QIAmp DNA stool mini kit (Qiagen, Hilden, Germany) according to the manufacturers protocol. DNA Purity and Concentration: The genomic DNA yields were estimated using measurement of absorbance at 260nm and 280nm using a spectrophotometer. The ratio of absorbance at 260nm to 280nm determines the purity of the genomic DNA yielded. According to the manufacturers protocol, the ratio of 1.7-1.9 indicates that the DNA yields are pure. PCR Typing by STS Primers: The genotype of B. hominis was determined by using PCR amplification with subtype-specific sequence tagged site (STS) primers (SB83, SB155, SB227, SB332, SB340, SB336, and SB337). The PCR reaction mixtures (20  µl of total volume) consisted of PCR buffer, 2.5 U/ µl of Taq DNA polymerase (Fermantas, SB38), 1.5 mM MgCl2, 1.25  µM of dNTPs (Fermantas, RO191), 0.5 pmol of forward primer, 0.5 pmol of reverse primer, and 1.0  µl of the DNA samples. In addition, the PCR conditions were set as follows: one cycle denaturing at 94 °C for 3 min, 30 cycles including denaturing at 94 °C for 30 seconds, annealing at 56.3 °C for 30 seconds, extending at 72 °C for 60 s, , and additional cycle with a 10 min chain elongation at 72 °C. The PCR products obtained were then electrophoresed in 1.5% agarose gel with 1X Tris-boric-EDTA (TBE) buffer. The fragments of the DNA were visualized using UV illuminator under UV light. The fragment sizes of the genomic DNA were confirmed with bands of a DNA ladder (50-1,000 bp DNA markers, Fermentas). Cultivation and collection of HCT116 Human colorectal carcinoma cell line, HCT116, was cultured in 25 cm ³ culture flask containing 5 ml of RPMI 1640 growth medium, supplemented with 5% of FBS, 2mM L-glutamine, 100 U/ml penicillin-streptomycin, and 2.5 ÃŽ ¼g/ml fungizone. The HCT116 cell line was then incubated in a CO2 incubator set at 100% humidity, atmosphere containing 5% of CO2 and a temperature of 37 °C. Prior to the introduction of antigen from Blastocystis hominis isolates, the cells were harvested from the substratum of the culture flask by using 0.25% trypsin-EDTA. The harvested cells were then washed with phosphate-buffered saline (PBS) thrice and resuspended in RPMI 1640 growth medium before introducing the solubilized antigen of Blastocystis hominis from symptomatic and asymptomatic isolates. Isolation of peripheral blood mononuclear cells (PBMCs) PBMCs were collected from fresh human blood sample (12 ml) in sterile EDTA tubes. The PBMCs were then isolated from the blood sample by using Histopaque ®-1077 (Sigma-Aldrich, USA) according to the density gradient centrifugation method (Boyum. 1974). The isolated PBMCs were then washed with PBS thrice and resuspended in 5 ml of RPMI 1640 growth medium, supplemented with 10% of FBS, 2mM L-glutamine, 100 U/ml penicillin-streptomycin, and 2.5 ÃŽ ¼g/ml fungizone in a 25 cm ³ culture flask prior to the introduction of solubilized antigen of Blastocystis hominis isolates. Introduction of the solubilized symptomatic and asymptomatic derived Blasto antigen into PBMCs and colorectal cancer cell line, HCT116 Harvested HCT116 cells (1 x 103 cells per well) in 100 ÃŽ ¼l of RPMI growth medium with 5% FBS and freshly isolated PBMCs (5 x 104 cells per well) in 100 ÃŽ ¼l of RPMI growth medium with 10% FBS were seeded into 96 well plates. After the overnight incubation in a CO2 incubator containing 5% CO2 at 37 °C, Blasto-antigen of B. hominis from symptomatic and asymptomatic individuals at final concentration ranging from 0.001 to 10ÃŽ ¼g/ml was added to each well containing the PBMCs and HCT116 cells and were further incubated for 48 hours. Then, the cell proliferation/viability was measured using the MTT assay (Mosmann. 1983). Quantitative Real-time Reverse Transcription Polymerase Chain Reaction (rtRT-PCR) Concentration of symptomatic and asymptomatic Blasto-antigen that yields the optimal proliferation and inhibition in the preliminary tests (MTT assay) was used to introduce into each culture flask containing PBMCs and HCT 116 cells respectively. For controls, PBS was introduced into both types of cells. After 48 hours of incubation, the RNA was isolated from the PBMCs and HCT 116 cells using Ambion RNAqueous Micro Kit (Ambion, CA, USA). The purified RNA obtained was then used to synthesize complementary DNA (cDNA) using High-Capacity RNA-to-cDNA kit (Applied Biosystems, USA) by the PCR method. Finally, Real-time reverse transcription PCR analysis was performed using inventoried primers (TaqMan ® Gene Expression Assays, Applied Biosystems). In this study, the genes of interest were IL-6, IL-8, tumour necrosis factor alpha (TNF-ÃŽ ±), nuclear factor kappa light chain enhancer of activated B cells (NF-ÃŽ ºB), interferon gamma (IFN-ÃŽ ³), protein 53 (p53), transforming growth factor be ta (TGF-ÃŽ ²), and cathepsin B (CTSB). The PCR reaction will be prepared according to the protocol that is generated by StepOneTM Software v2.0. Statistical Analysis In order to analyze the real-time RT-PCR gene expressions, the à ¢Ã‹â€ Ã¢â‚¬  CT value of the treated samples with symptomatic and asymptomatic Blasto-antigen was compared against the non-treated sample (control with PBS) using the Students t test. A P value of 0.05 was considered to be the minimum threshold of significance. RESULTS AND DISCUSSIONS In the present study, the symptomatic and asymptomatic isolates were genotyped by PCR amplification using the well-known seven sets of STS primers. The extracted DNA of symptomatic isolate showed positive amplification with SB83 and thus determined as subtype 1 (Yoshikawa et al. 2004). Nevertheless, the extracted DNA of asymptomatic isolate did not show any positive amplification with all the seven sets of STS primers that was used in this study. Hence, it is postulated that the asymptomatic isolate may be subtype 8 to 13 which was not amplified by the STS primers used in this study (Dominguez-Mà ¡rquez et al. 2009; Eroglu et al. 2009; Jones et al. 2009; Hussein et al. 2008; Tan et al. 2008). Molecular phylogenetic analysis need to be carried out in order to further characterized the subtype of asymptomatic isolate used in this study (Noel et al. 2005; Stensvold et al. 2007; Yoshikawa et al. 2007). In this study, the Blasto-Ag isolated from both symptomatic as well as asymptomatic isolates have caused an inhibition of PBMC cells proliferation which leads to the speculation that the Blasto-Ag may have caused apoptosis in the immune cells to prevent the propagation of immune cells to combat with the Blastocystis infection. The symptomatic isolate has caused a greater inhibition of PBMCs as compare to asymptomatic isolates. However, the Blasto-Ag mediated PBMCs cell inhibition is contrast to our previous report (Chandramathi et al. 2010). Our previous study showed that Blasto-Ag caused stimulation of PBMCs cell proliferation (Chandramathi et al. 2010). However, the molecular genotyping of the B. hominis isolates used in that study was not carried out. Past studies have reported that there may be a correlation between different B. hominis subtypes on the pathogenesis in human hosts (Dominguez-Mà ¡rquez et al. 2009; Eroglu et al. 2009; Jones et al. 2009; Hussein et al. 2008; Tan et al. 2008). This contrasting result observed in the cell viability of PBMCs may suggest the different subtypes of B. hominis may give different effects on the immune cells. Hence, further investigation is required to investigate the potential pathogenesis of different B. hominis subtypes. In the current study, increase in cell proliferation has been observed in colorectal carcinoma cell line, HCT116 cells upon exposure to antigen from symptomatic as well as asymptomatic isolates. HCT116 cells have shown significantly higher increase in cell proliferation stimulated by symptomatic Blasto-Ag as compared to asymptomatic antigen. The increase in the cell proliferation may further suggest that Blastocystis infection could facilitate the growth of colorectal cancer cells (Chandramathi et al. 2010). It is evident from the present study that symptomatic Blasto-Ag is more pathogenic as compared to asymptomatic Blasto-Ag. Hence, it may be essential to treat the Blastocystis infection in symptomatic colorectal cancer patients for better prognosis. On the other hand, a diverse pattern of cytokine, nuclear transcription factor as well as apoptotic gene expressions were observed upon the stimulation of HCT116 cells and PBMCs by symptomatic and asymptomatic derived Blasto-Ag. In the present study a noticeable up-regulation of Th1 (IFN-ÃŽ ³ and TNF-ÃŽ ±) and Th2 (IL6, IL8 and TGF-ÃŽ ²) cytokines was observed in HCT116 following exposure to symptomatic Blasto-Ag may lead to the speculation that B. hominis has activated the cellular and humoral immune responses in clearing the Blastocystis infection. This is accordance to the previous study that extracellular parasites such as B. hominis would be more effective in counteracted by a combination of Th1 and Th2 cytokines (Daugelat et al. 1996). Nevertheless, a more significant up-regulation of Th2 cytokines as compared to Th1 cytokines observed in HCT116 upon exposure to symptomatic and asymptomatic Blasto-Ag may lead to the postulation that symptomatic Blasto-Ag may resulting in a Th2- dominated responses and has the potential in weakening the cellular immune response, allowing the progression and growth of an existing tumour cells. On the other hand, HCT116 exposed to symptomatic Blasto-Ag has resulted in a more noticeable up-regulation of Th2 cytokines then asymptomatic Blasto-Ag which may lead to the speculation that asymptomatic Blasto-Ag is less pathogenic than symptomatic derived Blasto-Ag. Furthermore, the up-regulation of TGF-ÃŽ ² in the colorectal cancer cells has the potential role of anti-inflammatory and resulting in host immunosuppression by inhibiting the cell mediated immune responses against the tumour cells (Zou, 2005; Seruga et al. 2008). In addition, it is widely-accepted that humoral immunity is activated by extracellular microbes such as bacteria (Romagnani, 1996). B. hominis, being an extracellular allergen, has triggered the activation of host humoral immune responses which is evident by the significant up-regulation of Th2 cytokines in the PBMCs upon exposure to symptomatic and asymptomatic Blasto-Ag. Moreover, this results can be supported by the recent finding which shown an elevation of IL6 and IL8 level in the monocytes of healthy volunteers exposed to Leishmania infection (Menezes et al. 2008). In spite of that, an interesting finding has been observed in PBMCs stimulated by symptomatic and asymptomatic Blasto-Ag, besides activating the humoral immune responses, PBMCs exposed by Blasto-Ag has also triggered the activation of cellular mediated immune response characterized by Th1 cytokines. As described earlier, extracellular parasites may be more effective counteracted by a combination of Th1 and Th2 cytoki nes mediated immune responses (Daugelat et al. 1996). However, the activation of cellular mediated immune responses which is reflected by a significant up-regulation of TNF-ÃŽ ± and IFN-ÃŽ ³ may lead to the speculation that Blasto-Ag isolated from symptomatic as well as asymptomatic individual has the ability in causing extensive inflammatory damage in the host tissue as a result of macrophage mediated responses towards the parasitic infections. In addition, an up-regulation of NF-ÃŽ ºB and pro-inflammatory cytokines IL6 and IL8 was observed in HCT116 stimulated by symptomatic Blasto-Ag. Furthermore, these findings were also observed in PBMCs stimulated by symptomatic and asymptomatic Blasto-Ag. Nevertheless, HCT116 exposed to asymptomatic Blasto-Ag has exhibited an insignificant down-regulation of NF-à Ã‚ ºB gene expression which then leads to a significant lower expression in IL6 and IL8 as compared to HCT116 stimulated by symptomatic Blasto-Ag. Although the previous reports by Chandramathi et al. 2010 has stated the potential role of asymptomatic Blasto-Ag in causing the up-regulation of NF-à Ã‚ ºB and pro-inflammatory cytokines IL6 and IL8, however, the similar trend was not observed in the current study. As described earlier, different Blastocystis subtypes may have different effects on HCT116 cells as well as causing different gene expression profile in HCT116. To further characterize the gene expression profiles a s well as pathogenic potential of each subtype on HCT116 and PBMCs, more samples on different subtypes need to be recruited for the future study. Apart from this, NF-à Ã‚ ºB is also related to the apoptotic mediator genes namely p53 and CTSB. NF-à Ã‚ ºB has been postulated to play a role in inhibiting the apoptosis; hence it is hypothesized that the down-regulation of NF-à Ã‚ ºB gene expression will lead to the up-regulation of p53 and vice versa (Baldwin, 2001; Chwieralski et al. 2006). Although the recent study has suggested that CTSB may contribute to the cell apoptosis, however the studies have only reported on CTSB-mediated apoptosis in breast cancer cells as well as hepatocytes apoptosis in fulminant hepatic failure (Sandes et al, 2007; Yan et al. 2009). Nevertheless, the possibility of CTSB participation in the apoptosis of colorectal cancer cells has not been investigated. Moreover, there are a number of studies have shown that over expression of CTSB mRNA and elevation of its protein activity have been associated with the invasive and metastasis properties of various cancers (Campo et al. 1994; Yan et al, 1998; Hirai et al. 1999; Sanjeeva et al. 2001). The similar findings were observed in the current study where the HCT116 cells stimulated by symptomatic as well as asymptomatic Blasto-Ag have caused a significant up-regulation of CTSB. The over expression of CTSB in HCT116 cells stimulated by symptomatic and asymptomatic Blasto-Ag may implicate the potential of Blasto-Ag in causing the invasive and metastasis of colorectal cancer (Campo et al. 1994; Yan et al. 1998; Dora et al. 2003). In the present study, the insignificant down-regulation of p53 gene expressions observed in PBMCs may indicates that the symptomatic and asymptomatic Blasto-Ag may have prevented the PBMC cells to undergo the apoptosis process. The observation of p53 gene expressions obtained was contrary to the expectation. This may be due to the latent effects of the host immunity to down-regulate the apoptotic mediator gene in order to combat with the invasive B. hominis as well as to prevent persistent inflammation which can lead to carcinogenesis. Although the down-regulation pattern was observed, it does not causing a significant effect (0.95 and 0.96 fold decrease respectively). In contrast to PBMCs, stimulation of symptomatic Blasto-Ag in HCT116 has insignificantly down-regulated the gene expressions of p53. The insignificant down-regulation observed in HCT116 stimulated by symptomatic Blasto-Ag may be explained that it could be the reason that HCT116 cells to have low level of NF-à Ã‚ ºB gene expression level (1.34 fold increase). Nevertheless, the down-regulation observed may further suggest that symptomatic Blasto-Ag may has enhance the proliferation and progression of existing tumour besides causing an extensive release of pro-inflammatory cytokines. On the other hand, an interesting finding was observed in HCT116 exposed to asymptomatic Blasto-Ag, instead of causing down-regulation of p53 gene expression, asymptomatic Blasto-Ag has caused an up-regulation of p53 (1.09 fold increase) in HCT116. The findings and observations obtained from the current study are still far to characterize the mechanism of the potential pathogenic role of B. hominis in PBMCs as well as HCT116 cells. However, the current study was able to give an overview of the potential pathogenesis of symptomatic and asymptomatic derived Blasto-Ag in HCT116 and PBMCs. Moreover, the findings also suggest that symptomatic derived Blasto-Ag is more pathogenic as compare to asymptomatic Blasto-Ag. More studies still need to be done on the association of this emerging parasite on the association with the colorectal cancer. CONCLUSION The MTT cell proliferative assay and gene expression profile in this study has shown that the solubilized antigen isolated from symptomatic and asymptomatic individuals, at a particular concentration, could facilitate the proliferation and growth of colorectal carcinoma while having the ability to induce apoptosis on PBMCs immune cells. Moreover, the cell proliferative assay also successfully reported that antigen isolated from symptomatic individual is more pathogenic as compare to asymptomatic isolates, as it causes a significantly higher increase in cell proliferation of HCT116 colorectal carcinoma cell lines and a significantly higher increase in inhibition of PBMCs immune cells. Moreover, symptomatic isolate of B. hominis has exhibited a more evident increase in the gene expression of Th1 and Th2 cyokines. Hence, there is a vital need to screen colorectal cancer patients for B. hominis infection.